Isolation, morphological and molecular identification of Dunaliella species in Hoz-e Soltan and Aran-va-Bidgol salt Lakes (Iran)

Authors

  • Farkhondeh Saba MSc Graduate, Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran; Department of Plant Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  • Mahshid Sedghi MSc Graduate, Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran, Iran
  • Mohammad Ali Amoozegar Associate Prof., Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran; Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran, Iran
  • Seyed Abolhassan Shahzadeh Fazeli Associate Prof., Microorganisms Bank, Iranian Biological Resource Center (IBRC), (ACECR), Tehran; Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
Abstract:

In this study, Dunaliella has been isolated in Jun. 2014 from Hoz-e Soltan and Aran-va-Bidgol salt Lakes (Iran). The isolates have been identified morphologically with the aid of simple light and invert microscope after isolation and purification on their specific medium (Moh202). By using molecular techniques such as polymerase chain reaction (PCR) and amplification of 18S rDNA gene primers, further identification was done and morphological identification were confirmed. The isolation and molecular identification of the Dunaliella species from above-mentioned lakes revealed that, at least two species of Dunaliella, namely, D. parva and D. viridis exist in the studied area.

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Journal title

volume 17  issue 1

pages  70- 77

publication date 2016-08-22

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